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Digital Spectrophotometer is laboratory equipment which is used for quantitative analysis of various components in sample. It is most commonly utilized in biochemistry analysis based on DNA, RNA, & protein isolation. This spectrophotometer works on principle of Lambert-Beer Law and uses visible light of known wavelength. Digital Spectrophotometer is required for measuring amount of light absorbed by solute in order to detect its actual amount in sample. It comprises a light source, monochromator, aperture, photoresistor, and amplifier.